The auvricular cartilage grafts have been widely used in replacement of the temporomandibular joint disk. Cartilage grafts itself have a low metabolism and high survival rate after grafting. In processing the grafting materials, it was important
to
preserve the properties of chondrocyte proper. We used 15% glycerol and 10% DMSO (Dimethyl Sulfoxide) solutions for cartilage fixation before deep freezing.
We have performed the allogenic auricular cartilage graft in the tempormandibular joint of 20 rabbits which 10 specimen was treated with 15% glycerol and the other 10 specimen was treated with 10% DMSO respecitvely and examined in 1, 2, 4, 6 and
8
weeks
after operation histopathologically.
@ES The result were:
@EN 1. Inflammatory cell infitration around the grafted mater5ial appeared more glycerol groups than DMSO groups at 1 week, but each group has no differences after 2 weeks.
2. Degenerative changes of grafted auricular chondrocytes were more deveolped in glycerol group than DMSO groups till 4 weeks, but there were no differeces between two groups after 6 weeks.
3. Fibrous union between grafted fragment and mandibular condyle was prominent in DMSO group.
4. Vascular proliferation of the grafted auricualr carlilage was more developed in DMSO groups than glycerol group in early stage.
5. Amount of the additional growth of grafted auricular cartilage was more existed in DMSO groups than glycerol group.
6. General survival rate after grafting was more prominent in DMSO group.
In summary, allogenic auricular cartilage grafts treated with 15% glycerol and 10% DMSO solution lave supported to survivalbility as a cryopereservative agents, especially DMSO groups have little inflammatory cell infiltration in early stages and
degenerative changes and additional growth are more prominent than glycerol groups.
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